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This program will search for ORFs and TATA box in Eukaryotic DNA sequences. To perform a search please upload or paste your sequence at the text area provided and press the Send button.


Help: Any letters not corresponding to nucleotides,any numbers, spaces, star symbols (*) and brackets (){}[] will be removed from the input sequence. This program will search for Open Reading Frames in a Eukaryotic DNA sequence. It will also look for the presence of four - TATA boxes (TATATA, ATATAT, ATATAA and TATAAA), intron borders (5' GTAAGT or 5' GTAGGT and 3' AC) and Pyrimidine Rich regions of 15 up to 50 A and T nucleotides.. It will also search for all STOP (TAG, TAA, and TGA) codons in all 6 reading frames of the sequence. The program will identify ORFs longer than 300 nt. In addition, we use the Fickett's function for prediction of protein coding regions.

Uploading sequence from txt file: Upload your file with your sequence using the form provided. Notice only TXT files will be accepted. Then press the Send button. The uploaded sequence can be also edited while being in the text box. Any sequence already present in the text box prior the upload will be replaced from the uploaded one.

Enter DNA sequence:

                                                                                                                               [Sample sequence]


Created together with Dr. D. Kaloudas. For any comments or inquires you can email him...

This applet is published in the International Journal of Bioinformatics Research and Applications.