gRNA generator for Cas9 system:

This program is designed to identify Proto-spacer Adjacent Motifs (PAM, 5'-NGG-3') and to generate gRNA sequences. You can use the text area provided and by copying and pasting your sequence there (you can also name the input sequence). Apart from this, you can select your genomic (target) DNA from the provided 25 bacterial genome sequences. For this aim, please use the drop-down menu to select your input genome and the strand you like to search in. If you are looking at a bacterial genome please define the region.

Sequence from text area(5'-3') Escherichia coli K-12 (U00096) Staphylococcus aureus NCTC 8325 (NC_007795.1) Frischella perrara PEB0191 (NZ_CP009056) Dichelobacter nodosus VCS1703A (NC_009446.1) Acidilobus saccharovorans (NC_014374.1) Ignicoccus hospitalis KIN4/I (NC_009776.1) Thermofilum carboxyditrophus (CP007493.1) Metallosphaera sedula CuR1 (CP008822.1) Desulfurococcus fermentans DSM 16532(NC_018001.1) Acidianus hospitalis W1 (NC_015518.1) Geoglobus acetivorans SBH6 (NZ_CP009552.1) Methanosphaera stadtmanae DSM 3091 (NC_007681.1) Methanosarcina mazei Goe1(NC_003901.1) Mycobacterium tuberculosis KIT87190 (NZ_CP007809.1) Corynebacterium urealyticum DSM7109 (NC_010545.1) Thermogladius cellulolyticus 1633 (NC_017954) Pyrolobus fumarii 1A (NC_015931.1) Thermosphaera aggregans DSM 11486 (NC_014160.1) Hyperthermus butylicus DSM 5456(NC_008818.1) Methanococcus aeolicus Nankai-3(NC_009635.1) Corynebacterium pseudotuberculosis C231(NC_017301.1) Cellulophaga baltica 18 (NZ_CP009976.1) Chlorobium tepidum TLS (NC_002932.3) Chlamydia pneumoniae CWL029 (NC_000922.1) Helicobacter pylori BM012B (NZ_CP007605.1) 5' 3'

DNA region from position to bp



Sequence Name:

Please paste DNA sequence in a 5' to 3' orientation.