Dear Visitor,

Welcome to the Essential Bioinformatics Web Services (EBWS with 9 applications) and to the Extended Bioinformatics Web Services (EXBWS with 6 applications). There are 15 available bioinformatics applications on this page. You can compute your reverse complementary DNA sequence, generate random DNA, RNA or peptide oligomers, search for motifs in DNA, RNA or peptide sequences, perform digestions, find ORFs in prokaryotic or in eukaryotic DNA sequences, generate random DNA mutations, calculate DNA and RNA melting temperature, PCR annealing temperature, and hydropathy plots of proteins. You can also visit several bioinformatics websites.

There is also here a bioinformatics database on bacterial riboswitches as antibacterial drug targets called RSwitch and programs for computational design of allosteric ribozymes.

You can also check out your current IP address, build a calendar for any month of interest, and employ a scientific calculator.

When you use the first 9 bioinformatics applets, included in EBWS, please cite the paper by Dimitrios Kaloudas, Nikolet Pavlova, Robert Penchovsky- EBWS: Essential Bioinformatics Web Services for Sequence Analyses (2018) published in the journal of IEEE Transactions on Computational Biology and Bioinformatics. That research is funded by grant DN13/14/20.12.2017 awarded by the Bulgarian National Science Fund (BNSF).

When using the last 6 bioinformatics applets, included in EXBWS, please cite the paper by Robert Penchovsky, Nikolet Pavlova, Dimitrios Kaloudas - ExBWS: Extended Bioinformatics Web Services for Sequence Analyses in the International Journal of Bioinformatics Research and Applications. That research is funded by grant KP-06-H31/18/13.12.2019 awarded by the Bulgarian National Science Fund (BNSF).

A research paper on the RSwitch database by Robert Penchovsky, Nikolet Pavlova, Dimitrios Kaloudas - RSwitch: a novel bioinformatics database on riboswitches as antibacterial drug targets (2020) is published in the journal of IEEE Transactions on Computational Biology and Bioinformatics. That research is funded by grant DN13/14/20.12.2017 awarded by the Bulgarian National Science Fund (BNSF).

We have created two computer programs (see the images below) for sequence generation of hammerhead allosteric ribozymes (a oligonucleotide-sensing ribozyme generator - RG, and an inverse ribozyme generator - IFRG) with YES Boolean logic function that work under Linux/Unix. They are based on algorithms published in Nature Biotechnology and are written in Perl/Tk. The programs are free to use for academic research and you can apply for them by contacting us.

We also have a new paper on computer design of high-speed allosteric ribozymes (see the images below) published in the Journal of Biotechnology, July 2023. The paper presents two programs for the computer design of fast allosteric ribozymes with randomized oligonucleotide-binding sites with YES (GHOST-YES) or NOT (GHOST-NOT) Boolean logic functions. This software is under the terms of the GNU General Public License as published by the Free Software Foundation; either version 3 of the License, or at your option any later version. GNU General Public License v3.0 form - GNU Project - Free Software Foundation. That research is funded by grant KP-06-H31/18/13.12.2019 awarded by the Bulgarian National Science Fund (BNSF). This research is published in Computers in Biology and Medicine.For the programs' installation guide click here. The programs are free to use for academic research and you can apply for them by contacting us.