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Input sequence size: = 34 bp

Your input sequence:
5'- GGATCCTGCGACCTGCAGGCATGCAAGCTTGGCA -3'

Reverse complement:
5'- UGCCAAGCUUGCAUGCCUGCAGGUCGCAGGAUCC -3'


TM calculation for: DNA/RNA


Melting temp.(TM) =89.76oC

CG content: 61.76 %

Oligo Conc. 10000 nM

Na+ Salt Conc. 500 mM


Thermodynamic properties

Enthalpy (ΔH) = -301.25 kcal/mol

Entropy (ΔS) = -804.47 kcal/mol



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